Unit of Competency Mapping – Information for Teachers/Assessors – Information for Learners

MSL975033 Mapping and Delivery Guide
Perform tissue and cell culture techniques

Version 1.0
Issue Date: May 2024


Qualification -
Unit of Competency MSL975033 - Perform tissue and cell culture techniques
Description
Employability Skills
Learning Outcomes and Application This unit of competency describes the skills and knowledge to prepare, maintain and preserve cells and cell lines for a variety of applications, such as large scale culture, production of monoclonal antibodies, production of viral vaccines and amniocentesis studies. Personnel are required to optimise equipment set-up, media and growth techniques. They are required to detect and investigate contamination and take preventative and/or corrective actions under supervision.This unit applies to laboratory technicians and technical officers working in laboratories in the biomedical, environmental, biotechnology and education industry sectors. No licensing or certification requirements exist at the time of publication. However, regulations and/or external accreditation requirements for laboratory operations exist, so local requirements should be checked. Relevant legislation, industry standards and codes of practice within Australia must also be applied.
Duration and Setting X weeks, nominally xx hours, delivered in a classroom/online/blended learning setting.
Prerequisites/co-requisites
Competency Field Testing
Development and validation strategy and guide for assessors and learners Student Learning Resources Handouts
Activities 		Slides
PPT 		Assessment 1 Assessment 2 Assessment 3 Assessment 4
Elements of Competency Performance Criteria              
Element: Maintain records
  • Maintain records of batches of media
  • Maintain records of active and stored tissue and cell lines
  • Complete records so that they are retrievable, legible and accurate
  • Ensure records conform to the information management, records, quality system and legal requirements
       
Element: Interpret and schedule production requirements
  • Review client request and confirm quantity and nature of cells, tissue or products
       
Element: Select appropriate media, materials, equipment and methods
       
Element: Plan parallel work sequences to optimise production
       
Element: Maintain a chain of custody, traceable to the worker, for all cells and tissues
       
Element: Work safely according to the legal and regulatory framework
  • Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and equipment
       
Element: Select, fit and use personal protective equipment (PPE)
       
Element: Address hazards and incidents as they arise
       
Element: Safely dispose of biohazardous materials and other laboratory waste
       
Element: Assemble and maintain tissue culture equipment
  • Assemble, sterilise or decontaminate equipment according to workplace procedures
       
Element: Perform pre-use and safety checks in accordance with relevant workplace and operating procedures
       
Element: Identify faulty or unsafe components and equipment and report to appropriate personnel
       
Element: Decontaminate area and equipment after use
       
Element: Prepare and test cell and tissue culture media
  • Confirm media specifications and processes/methods
       
Element: Prepare culture media to suit client request
       
Element: Sterilise culture media and check for sterility
       
Element: Perform quality control checks to ensure that culture media is fit for purpose
       
Element: Store culture media in accordance with specifications
       
Element: Obtain, monitor and maintain tissue and cell lines
  • Retrieve/obtain the cell lines or tissue sample from fresh or preserved sources and prepare a culture
       
Element: Select specified culture media and add any necessary growth agents or nutrients
       
Element: Incubate cells/tissue in specified conditions
       
Element: Inoculate the media with the specified amount of sample
       
Element: Monitor growth of tissue and cell lines and products
       
Element: Detect contamination/abnormalities and troubleshoot materials, equipment and techniques
       
Element: Passage samples by subculturing to preserve or grow the line
       
Element: Harvest cells or cell products to optimise yields
       
Element: Preserve cells and tissues
  • Select the appropriate preservation method
       
Element: Preserve the cell lines or tissue in accordance with the method
       
Element: Check preserved cell lines regularly to ensure viability is maintained
  • Maintain records of batches of media
       
Element: Maintain records of active and stored tissue and cell lines
       
Element: Complete records so that they are retrievable, legible and accurate
       
Element: Ensure records conform to the information management, records, quality system and legal requirements
       

Submission Requirements

List each assessment task's title, type (eg project, observation/demonstration, essay, assignment, checklist) and due date here

Assessment task 1: [title]      Due date:

(add new lines for each of the assessment tasks)

Assessment Tasks

Copy and paste from the following data to produce each assessment task. Write these in plain English and spell out how, when and where the task is to be carried out, under what conditions, and what resources are needed. Include guidelines about how well the candidate has to perform a task for it to be judged satisfactory.
Copy and paste from the following performance criteria to create an observation checklist for each task. When you have finished writing your assessment tool every one of these must have been addressed, preferably several times in a variety of contexts. To ensure this occurs download the assessment matrix for the unit; enter each assessment task as a column header and place check marks against each performance criteria that task addresses.

Observation Checklist

Tasks to be observed according to workplace/college/TAFE policy and procedures, relevant legislation and Codes of Practice Yes No Comments/feedback
Maintain records of batches of media 
Maintain records of active and stored tissue and cell lines 
Complete records so that they are retrievable, legible and accurate 
Ensure records conform to the information management, records, quality system and legal requirements 
Review client request and confirm quantity and nature of cells, tissue or products 
 
 
 
Identify hazards and workplace control measures associated with the sample, preparation methods, reagents and equipment 
 
 
 
Assemble, sterilise or decontaminate equipment according to workplace procedures 
 
 
 
Confirm media specifications and processes/methods 
 
 
 
 
Retrieve/obtain the cell lines or tissue sample from fresh or preserved sources and prepare a culture 
 
 
 
 
 
 
 
Select the appropriate preservation method 
 
Maintain records of batches of media 
 
 
 

Forms

Assessment Cover Sheet

MSL975033 - Perform tissue and cell culture techniques
Assessment task 1: [title]

Student name:

Student ID:

I declare that the assessment tasks submitted for this unit are my own work.

Student signature:

Result: Competent Not yet competent

Feedback to student

 

 

 

 

 

 

 

 

Assessor name:

Signature:

Date:

Assessment Record Sheet

MSL975033 - Perform tissue and cell culture techniques

Student name:

Student ID:

Assessment task 1: [title] Result: Competent Not yet competent

(add lines for each task)

Feedback to student:

 

 

 

 

 

 

 

 

Overall assessment result: Competent Not yet competent

Assessor name:

Signature:

Date:

Student signature:

Date: